| Catalog No. | Size | Price (USD) |
| 11159-HNAH-1 | 1 mg | $2,474.19 |
| 11159-HNAH-100 | 100 µg | $573.19 |
| 11159-HNAH-200 | 200 µg | $573.19 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested). |
| Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized CTLA-4 Protein, Human, Recombinant (Cat:11159-HNAH) at 2 μg/mL (100 μL/well) can bind B7-1 Protein, Human, Recombinant (His & hFc Tag), Biotinylated, the EC 50 of B7-1 is 6-27 ng/mL. 2.Immobilized Recombinant Human CTLA-4 / CD152 Protein (Cat: 11159-HNAH) at 2 μg/mL (100 μL/well) can bind Anti-CTLA4(BMS) Antibody, Human IgG1, the EC 50 is 2.5-7.5 ng/mL (Routinely tested). 3.Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein (Cat. No. 11159-HNAH) with an affinity constant of 2.09 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Loaded Recombinant Human B7-2/CD86 Protein, hFc Tag (Cat. No. 10699-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein (Cat. No. 11159-HNAH) with an affinity constant of 5.17 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the human CTLA4 (NP_005205.2) (Met1-Asp161) was expressed. |
| Accession# | NP_005205.2 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Lys 36 |
| Molecule Mass | The recombinant human CTLA4 consists of 126 amino acids and predicts a molecular mass of 13.5 kDa. The molecular weight of this protein is around 34.4 kDa verified by SEC-MALS(Routinely tested). |
Synonyms: ALPS5 Protein, Human; CD Protein, Human; CD152 Protein, Human; CELIAC3 Protein, Human; CTLA-4 Protein, Human; GRD4 Protein, Human; GSE Protein, Human; IDDM12 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human CTLA-4/CD152 Protein 14114 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human CTLA-4 Protein 30790 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human CTLA-4 Protein 38652 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human CTLA-4 Protein 40599 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human CTLA-4 Protein 40988 |
| Image 5 URL | Source image |
Background Information
| Full Name | cytotoxic T-lymphocyte-associated protein 4 |
| Description | Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg). |
| Research Areas |
|
| Tags |
|
| References |
|
title: In vitro characterization of a bispecific anti-(PD-1/CTLA-4) single-chain diabody for cancer immunotherapy
authors: Mohammad Ghoreishi; Nafiseh Esmaeil; ...; Maryam Abbaspour
journal: Scientific Reports
date: 2026-06-05
pmid: 42248962
doi: 10.1038/s41598-026-55919-6
cited_by_count: 1
techniques: Affinity Chromatography; Binding Assay; Construct; Control; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; High Molecular Weight; Incubation; Marker; Molecular Weight; Negative Control; Plasmid Preparation; Positive Control; Purification; Recombinant; SDS Page; Size-exclusion Chromatography; Western Blot
bioz_score: 94
title: Discovery and characterization of two anti-PD-1 antibodies with a unique binding mechanism to human PD-1
authors: Keyla María Gómez Castellano; Alejandra Montes Luna; ...; Juan Carlos Almagro
journal: mAbs
date: 2025-11-25
pmid: 41292073
doi: 10.1080/19420862.2025.2591461
cited_by_count: 0
techniques: Binding Assay; Control; Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 93
title: Botensilimab, an Fc-Enhanced Anti–CTLA-4 Antibody, Is Effective against Tumors Poorly Responsive to Conventional Immunotherapy
authors: Dhan Chand; David A. Savitsky; ...; Robert B. Stein
journal: Cancer Discovery
date: 2024-12-02
pmid: 39083809
doi: 10.1158/2159-8290.CD-24-0190
pmcid: 11609826
cited_by_count: 18
techniques: Binding Assay; Injection; Recombinant; SPR Assay
bioz_score: 93
title: Preclinical imaging evaluation of a bispecific antibody targeting hPD1/CTLA4 using humanized mice.
authors: Xingguo Hou; Song Liu; ...; Zhi Yang
journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
date: 2024-04-26
pmid: 38677243
doi: 10.1016/j.biopha.2024.116669
cited_by_count: 7
techniques: Binding Assay; Construct
bioz_score: 93
title: FcγRIIB Is an Immune Checkpoint Limiting the Activity of Treg-Targeting Antibodies in the Tumor Microenvironment
authors: David A. Knorr; Lucas Blanchard; ...; Jeffrey V. Ravetch
journal: Cancer Immunology Research
date: 2024-03-04
pmid: 38147316
doi: 10.1158/2326-6066.CIR-23-0389
pmcid: 10911703
cited_by_count: 23
techniques: Activity Assay; Binding Assay; Blocking Assay; Comparison; Competitive ELISA; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Fluorescence; Incubation; Isolation; MANN-WHITNEY; Recombinant; SPR Assay
bioz_score: 93
title: HOW I DIAGNOSE AND TREAT ANTIPHOSPHOLIPID SYNDROME IN PREGNANCY.
authors: D Ware Branch; Ming Y Lim
journal: Blood
date: 2023-12-25
pmid: 38145574
doi: 10.1182/blood.2023020727
cited_by_count: 10
techniques: Activity Assay; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Mass Spectrometry; Purification; Recombinant; SDS Page; Staining
bioz_score: 93
title: FcyRIIB is a novel immune checkpoint in the tumor microenvironment limiting activity of Treg-targeting antibodies
authors: Knorr David; Leidner Rom; ...; Ravetch Jeffrey
journal: bioRxiv
date: 2023-01-20
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Fluorescence; Generated; In Vivo; Incubation; Recombinant; SPR Assay; Sequencing; Variant Assay
bioz_score: 93
title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways.
authors: Yunlong Zhao; Calvin K Lee; ...; Enfu Hui
journal: Immunity
date: 2020-01-30
pmid: 31757674
cited_by_count: 215
techniques: Cell Isolation; Cross-linking Immunoprecipitation; Enzyme-linked Immunosorbent Assay; Imaging; Immunopeptidomics; Isolation; Recombinant; Software
bioz_score: 93
title: A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy
authors: Xuexiang Du; Fei Tang; ...; Yang Liu
journal: Cell Research
date: 2018-02-22
pmid: 29472691
doi: 10.1038/s41422-018-0011-0
pmcid: 5939050
cited_by_count: 177
techniques: Activity Assay; Binding Assay; Blocking Assay; Cell Culture; Concentration Assay; Expressing; Flow Cytometry; Fluorescence; Functional Assay; In Vitro; In Vivo; Incubation; Inhibition; Injection; Knock-In; Labeling; MANN-WHITNEY; Negative Control; Positive Control; Purification; Recombinant; SDS Page; Staining; Transfection
bioz_score: 93
