| Catalog No. | Size | Price (USD) |
| M23-11G-10 | 10 µg | $515.43 |
| M23-11G-50 | 50 µg | $1,729.93 |
| M23-11G-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~65 kDa |
| Technical Data Sheet by Lot # | A4640-3 H2630-7 J3870-11 K4542-2 K4542-3 K4555-17 K4555-18 L5137-16 N3472-4 O3959-8 O3959-9 R2782-7 T4253-1 U4761-10 U4761-11 |
Synonyms: HPK1 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases HPK1 (Cat. No.: M23-11G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human MAP4K1/HPK1 Protein 46445 |
| Image 1 URL | Source image |
title: Isoquinoline compounds and uses thereof
authors: Jun Liang; Sushant Malhotra; ...; Yonghan Hu
date: 2025-08-05
cited_by_count: 0
techniques: Binding Assay; Concentration Assay
bioz_score: 93
title: Naphthyridine compounds and uses thereof
authors: Terry Kellar; Jun Liang; ...; Yonghan Hu
date: 2025-05-13
cited_by_count: 0
techniques: Binding Assay; Concentration Assay
bioz_score: 93
title: Substituted indazoles as hematopoietic progenitor kinase 1 (HPK1) inhibitors
authors: Jinhwa Lee; Suyeon Jo; ...; Seung Mook Lim
date: 2023-12-05
cited_by_count: 0
techniques: Activity Assay; Incubation; Kinase Assay; Recombinant
bioz_score: 93
title: Pyrazolopyridine compounds and uses thereof
authors: Oleg Vechorkin; Jun Pan; ...; Wenqing Yao
date: 2023-10-24
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Solid forms of an HPK1 inhibitor
authors: Zhongjiang Jia; William Frietze
date: 2023-10-17
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Indazoles as hematopoietic progenitor kinase 1 (HPK1) inhibitors and methods using same
authors: Jinhwa Lee; Suyeon Jo; ...; Seung Mook Lim
date: 2023-05-16
cited_by_count: 0
techniques: Activity Assay; Incubation; Kinase Assay; Recombinant
bioz_score: 93
title: 8-aminoisoquinoline compounds and uses thereof
authors: Jun Liang; Rohan V. Mendonca; ...; Timothy Heffron
date: 2023-03-28
cited_by_count: 0
techniques: Binding Assay; Concentration Assay
bioz_score: 93
title: Isoquinolines as inhibitors of HPK1
authors: Bryan Chan; Joy Drobnick; ...; Andrew Good
date: 2023-01-31
cited_by_count: 0
techniques: Binding Assay; Concentration Assay
bioz_score: 93
title: Discovery of Pyrazolopyridine Derivatives as HPK1 Inhibitors
authors: Qinda Ye; Kai Liu; ...; Wenqing Yao
journal: ACS medicinal chemistry letters
date: 2022-12-12
pmid: pm36655125
cited_by_count: 0
techniques: Activity Assay; Concentration Assay; Incubation
bioz_score: 93
title: The HPK1 Inhibitor A-745 Verifies the Potential of Modulating T Cell Kinase Signaling for Immunotherapy.
authors: Sven Malchow; Alla Korepanova; ...; William N Pappano
journal: ACS chemical biology
date: 2022-04-29
pmid: 35188729
doi: 10.1021/acschembio.1c00819
cited_by_count: 13
techniques: Binding Assay; Incubation; Labeling
bioz_score: 93
title: Benzimidazole and indole compounds and uses thereof
authors: Joshua Hummel; Minh Nguyen; ...; Wenqing Yao
date: 2022-04-12
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Pyrazolopyrimidine compounds and uses thereof
authors: Oleg Vechorkin; Kai Liu; ...; Joshua Hummel
date: 2021-05-25
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Azaindoles as inhibitors of HPK1
authors: Timothy Heffron; Sushant Malhotra; ...; Yonghan Hu
date: 2021-03-23
cited_by_count: 0
techniques: Concentration Assay; Inhibition
bioz_score: 93
title: Benzothiazole compounds and uses thereof
authors: Joshua Hummel; Oleg Vechorkin; ...; Wenqing Yao
date: 2021-01-26
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Carboxamide compounds and uses thereof
authors: Oleg Vechorkin; Jun Pan; ...; Wenqing Yao
date: 2020-10-13
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Indazole compounds and uses thereof
authors: Alexander Sokolsky; Oleg Vechorkin; ...; Wenqing Yao
date: 2020-08-25
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Cyanoindazole compounds and uses thereof
authors: Oleg Vechorkin; Onur Atasoylu; ...; Wenqing Yao
date: 2020-07-28
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
title: Naphthyridines as inhibitors of HPK1
authors: Bryan Chan; Naomi Rajapaksa; ...; Rohan Mendonca
date: 2019-09-10
cited_by_count: 0
techniques: Binding Assay; Concentration Assay
bioz_score: 93
title: Pyrazolopyridone compounds and uses thereof
authors: Qinda Ye; Kai Liu; ...; Wenqing Yao
date: 2019-05-07
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 93
MDSD
