| Catalog No. | Size | Price (USD) |
| 40591-T62-B-20 | 20 µg | $177.73 |
| 40591-T62-B-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV-2 Spike Antibody (Biotin), Rabbit PAb, Antigen Affinity Purified |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike S1 |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike S-His Protein (Catalog#40591-V08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant SARS-CoV-2 (2019-nCoV) Spike S1-His Recombiant Protein (HPLC-verified) (Catalog#40591-V08H; YP_009724390.1; Val16-Arg685) and then biotinylated. The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Spike affinity chromatography and then biotinylated. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Label-free, non-optical detection of intact SARS-CoV-2 virions in native human saliva via microscale surface ion conduction.
authors: Sommer Osman; Sanduni U Devasinghe; ...; Robbyn K Anand
journal: Biosensors & bioelectronics
date: 2026-03-21
pmid: 41895232
doi: 10.1016/j.bios.2026.118643
cited_by_count: 0
techniques: Cell Culture; Virus
bioz_score: 94
title: Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces
authors: Rina Uchida; Ayuri Mitsuno; ...; Hiroaki Sakamoto
journal: Biochemical Engineering Journal
date: 2025-01-01
doi: 10.1016/j.bej.2024.109534
cited_by_count: 0
techniques: High Performance Liquid Chromatography; Recombinant
bioz_score: 93
title: A Single Vaccine Protects against SARS-CoV-2 and Influenza Virus in Mice.
authors: Kangli Cao; Xiang Wang; ...; Jianqing Xu
journal: Journal of virology
date: 2022-02-28
pmid: 34908443
doi: 10.1128/JVI.01578-21
cited_by_count: 24
techniques: Construct; Purification; Transfection; Western Blot
bioz_score: 94
