WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human CD200R
Immunogen
Recombinant Human CD200R1 protein (Catalog#11218-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD200R1 (rh CD200R1; Catalog#11218-H08H; AAI43394.1; Met1-Leu266). CD200R1 specific IgG was purified by Human CD200R1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
CD200R1 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5 µL anti-CD200R1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CD200R1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa
CD200R1 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5 µL anti-CD200R1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CD200R1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cell surface glycoprotein CD200 receptor 1 (CD200R1) is an isoform of CD200 receptors that is expressed on cells of the myeloid lineage. CD200R1 is a receptor for the OX-2 membrane glycoprotein. The receptor-substrate interaction may serve as a myeloid downregulatory signal.
Research Areas
Neuroinflammation
Cancer Drug Targets
Tags
Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.