Recombinant Anti-Cystatin D Antibody, Rabbit Monoclonal
Validated applications
ELISA (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human Cystatin D No cross-reactivity in ELISA with Human FetuinA Mouse AHSG Human CST3
Immunogen
Recombinant Human Cystatin D / CST5 protein (Catalog#10506-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin D / CST5 (rh Cystatin D / CST5; Catalog#10506-H08H; NP_001891.2; Met1-Val142).
Source
Monoclonal Rabbit IgG Clone #011
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Background Information
Full Name
cystatin D
Description
Cystatins are natural inhibitors of papain-like (family C1) and legumain-related (family C13) cysteine peptidases. The mammalian cystatin superfamily members are of three major types, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. As a member of type 2 cystatin, cystatin D is a single-domain protein and also has cysteine residues that form disulfide bridges. In contrast with the wider distribution of all the other family 2 cystatins, cystatin D is tissue-restricted expressed and has been found only in saliva and tears. and meanwhile, it displays an inhibition profile with a preferential inhibition on cathepsin S, H, L. Although the exact functions are largely unknown, it has reported that cystatin D is involved in the inhibition of virus replication and apoptosis.
References
Freije J.P., et al. ,(1991), Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor. J. Biol. Chem. 266:20538-20543.
Freije J.P., et al. , (1993), Human cystatin D. cDNA cloning, characterization of the Escherichia coli expressed inhibitor, and identification of the native protein in saliva.J. Biol. Chem. 268:15737-15744.
Deloukas P., et al. ,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.