| Catalog No. | Size | Price (USD) |
| 110472-R0010-20 | 20 µL | $103.53 |
| 110472-R0010-100 | 100 µL | $296.07 |
General Information
| Product name | Phospho-SMAD2 (Ser465, 467) Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Zebrafish |
| Specificity | Human Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Zebrafish |
| Immunogen | A synthetic peptide corresponding to the residues around (Ser465, 467) of Human Phospho-SMAD2 |
| Preparation | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Ser465, 467) of Human Phospho-SMAD2. |
| Source | Monoclonal Rabbit IgG Clone #0010 |
| Purification | Protein A |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CHTD8 Antibody; Anti-hMAD-2 Antibody; Anti-hSMAD2 Antibody; Anti-JV18 Antibody; Anti-JV18-1 Antibody; Anti-LDS6 Antibody; Anti-MADH2 Antibody; Anti-MADR2 Antibody; Anti-SMAD2 Antibody
Image / Experimental Information
| Image 1 Description | Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with TGF beta 1 (10804-HNAC) (5 ng/mL, 120min; +)(line B), using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody (Cat: 110472-R0010) at 1:1000 dilution (upper) or Anti-SMAD2 Antibody, Rabbit polyclonal (Cat: 50727-T58) at 1:100000 dilution (lower). |
| Image 1 Alt Text | Human Smad2 Western blot (WB) 42795 |
| Image 1 URL | Source image |
| Image 2 Description | Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with TGF beta 1 (10804-HNAC) (5 ng/mL, 120min; +)(line B), using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody (Cat: 110472-R0010) at 1:1000 dilution (upper) or Anti-SMAD2 Antibody, Rabbit polyclonal (Cat: 50727-T58) at 1:100000 dilution (lower). |
| Image 2 Alt Text | Human Smad2 Western blot (WB) 42796 |
| Image 2 URL | Source image |
| Image 3 Description | Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with TGF beta 1 (10804-HNAC) (5 ng/mL, 120min; +)(line B), using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody (Cat: 110472-R0010) at 1:1000 dilution (upper) or Anti-SMAD2 Antibody, Rabbit polyclonal (Cat: 50727-T58) at 1:100000 dilution (lower). |
| Image 3 Alt Text | Human Smad2 Western blot (WB) 42797 |
| Image 3 URL | Source image |
Background Information
| Full Name | SMAD family member 2 |
| Description | SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. |
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