| Catalog No. | Size | Price (USD) |
| 100321-T32-50 | 50 µL | $147.96 |
| 100321-T32-100 | 100 µL | $251.64 |
| 100321-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-IL-12 p35/IL12A Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse IL-12 p35/IL12A |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the mouse IL12A . |
| Preparation | Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the mouse IL12A . Mouse IL12A specific IgG was purified by Mouse IL12A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Il-12a Antibody; Anti-IL-12p35 Antibody; Anti-Il12a Antibody; Anti-Ll12a Antibody; Anti-p35 Antibody
Image / Experimental Information
| Image 1 Description | Anti-IL12A rabbit polyclonal antibody at 1:500 dilution Lane A: NIH-3T3 Whole Cell Lysate Lane B: Hu-T78 Whole Cell Lysate Lane C: Raw264.7 Whole Cell Lysate Lane D: MDA-MB-231 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Mouse IL12A / IL-12A Western blot (WB) 13072 |
| Image 1 URL | Source image |
Background Information
| Full Name | interleukin 12A |
| Description | Interleukin-12 subunit alpha (IL12A/IL-12p35) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 1, p35, NK cell stimulatory factor chain 1, and interleukin-12 alpha chain. IL12A/IL-12p35 is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A/IL-12p35 is required for the T-cell-independent induction of interferon (IFN)-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. In clinical, IL-12 remains a very promising immunotherapeutic agent because recent cancer vaccination studies in animal models and humans have demonstrated its powerful adjuvant properties. The immune-modulating characteristics of IL-12 considered responsible for the adjuvant effects, as well as the results of animal and human cancer vaccination studies with IL-12, applied as an adjuvant. IL12A/IL-12p35 indicates a cytokine that is important in the development of prostate cancer. |
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