Rat TNF-α ELISA kit CSB-E11987r-IS CUSABIO
$50.00
In stock
SKU
CSB-E11987r-IS
Product Details
| Uniprot No. | P16599 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit |
| Abbreviation | TNF |
| Sample Types | tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: cell culture supernates(n=4); 1: 1:1; 2: Average %; 3: 107; 1: Range %; 2: 99-113; 1: 1:2; 2: Average %; 3: 99; 1: Range %; 2: 96-105; 1: 1:4; 2: Average %; 3: 103; 1: Range %; 2: 97-109; 1: 1:8; 2: Average %; 3: 99; 1: Range %; 2: 94-102 |
| Recovery | headers: The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: cell culture supernates (n=5); 2: 94; 3: 89-100 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.717; 3: 2.634; 4: 2.675; 5: 2.487; 1: 500; 2: 1.476; 3: 1.467; 4: 1.471; 5: 1.283; 1: 250; 2: 0.853; 3: 0.908; 4: 0.881; 5: 0.693; 1: 125; 2: 0.608; 3: 0.629; 4: 0.619; 5: 0.431; 1: 62.5; 2: 0.395; 3: 0.381; 4: 0.388; 5: 0.200; 1: 31.2; 2: 0.281; 3: 0.293; 4: 0.287; 5: 0.099; 1: 15.6; 2: 0.218; 3: 0.207; 4: 0.213; 5: 0.025; 1: 0; 2: 0.191; 3: 0.185; 4: 0.188 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of TNF (tumor necrosis factor) in Rattus norvegicus samples, supporting immunology research focused on innate immune activation and inflammatory signaling. Because TNF is a rapidly induced, short-lived cytokine whose concentrations can vary dramatically between baseline and inflammatory states, this kit is designed to capture that full dynamic window across multiple sample contexts. Detection Range: The 15.6–1000 pg/mL range spans from near-baseline circulating TNF levels in unstimulated rat tissues to the elevated concentrations observed in LPS-challenged or sepsis-model animals, minimizing the need for serial dilutions while covering both resting and acutely inflamed conditions. Sensitivity: At 3.9 pg/mL, the kit detects TNF in samples where expression is low — such as unstimulated macrophage supernatants, early-phase inflammatory responses, or tissue homogenates from localized injury models — where TNF may be present but not yet amplified. Sample Compatibility: Validation across tissue homogenates, cell culture supernatants, and bronchoalveolar lavage fluid enables researchers to connect organ-level TNF production, cellular secretion kinetics, and pulmonary compartment inflammation within a single experimental framework, critical for acute lung injury or pneumonia models. Assay Efficiency: Requiring only 50–100 µL and 1–5 hours, the kit accommodates limited-volume BAL fluid collections and time-sensitive multi-cytokine profiling from primary rat macrophage cultures. This kit directly supports studies in experimental sepsis, ventilator-induced lung injury, and rheumatoid arthritis modeling. |
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