| Catalog No. | Size | Price (USD) |
| M25-11G-10 | 10 µg | $974.57 |
| M25-11G-50 | 50 µg | $3,062.93 |
| M25-11G-500 | 500 µg | $5,551.19 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant human GLK (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~70 kDa |
| Technical Data Sheet by Lot # | A4684-9 A4702-13 A4705-7 D2508-8 L1885-7 L1969-10 L5167-24 L5171-14 L5171-15 R2879-8 V2467-7 |
Synonyms: GLK Protein, Human; MAPKKKK3 Protein, Human; MEKKK 3 Protein, Human; MEKKK3 Protein, Human; RAB8IPL1 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases GLK (Cat. No.: M25-11G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human MAP4K3/GLK Protein 46427 |
| Image 1 URL | Source image |
Background Information
| Description | MAP4K3 (also named germinal-center kinase-like kinase, GLK) is a serine/threonine kinase, which belongs to the mammalian Ste20-like kinase family. MAP4K3 is required for robust cell growth in response to amino acids. MAP4K3 positively regulates preterm skeletal muscle satellite cell myogenesis, but may not regulate mTORC1 activity. MAP4K3 may play a role in mTORC1 full activation in response to leucine. MAP4K3 is required for phosphorylation of known mTOR targets such as S6K1 (S6 kinase 1), and overexpression of MAP4K3 promotes the rapamycin-sensitive phosphorylation of these same targets.MAP4K3 is a new component in the nutrient-responsive pathway for mTOR activation and reveals a completely new function for MAP4K3 in promoting cell growth. |
| References |
|
title: Epinephrine inhibits PI3Kα via the Hippo kinases
authors: Ting-Yu Lin; Shakti Ramsamooj; ...; Marcus D. Goncalves
journal: Cell Reports
date: 2024-01-10
pmid: 38060450
doi: 10.1016/j.celrep.2023.113535
pmcid: 10809223
cited_by_count: 1
techniques: Double Knockout; Fluorescence; Isolation; Mutagenesis; Plasmid Preparation; Recombinant; Software
bioz_score: 92
title: The HPK1 Inhibitor A-745 Verifies the Potential of Modulating T Cell Kinase Signaling for Immunotherapy.
authors: Sven Malchow; Alla Korepanova; ...; William N Pappano
journal: ACS chemical biology
date: 2022-04-29
pmid: 35188729
doi: 10.1021/acschembio.1c00819
cited_by_count: 13
techniques: Binding Assay; Expressing; Fluorescence; Purification; X-Ray Crystallography
bioz_score: 92
MDSD
