General Information
| Product name | Human IL-21 ELISA Kit |
| Specificity | This assay recognizes recombinant Human IL-21. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Recombinant protein |
| Sensitivity | 18.8 pg/mL |
| Assay range | 31.25-2000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This Human IL-21 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-21 protein in Recombinant protein . It contains recombinant Human IL-21, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
Synonyms: CVID11 ELISA Kit, Human; IL-21 ELISA Kit, Human; Za11 ELISA Kit, Human
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | Human IL-21 ELISA Kit Standard Curve 39421 |
| Image 1 URL | Source image |
Background Information
| Full Name | interleukin 21 |
| Description | IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation). |
| Related Pathways | - AKT Signaling Pathway
- Jak-Stat Signaling Pathway
- Autophagy Pathway
- Common G-Chain signaling pathway
- IL12 signaling pathway
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| Tags | - Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
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| References | - Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34.
- Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10.
- Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.
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